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ULTRA SENSITIVE, UNIFORM, FLUORESCENCE QUENCHING, FLEXIBLE & CUSTOMIZABLE SERS SUBSTRATES

About our SERS susbtrates

SERS substrates from Nefer Ka consist of metallic, Ag or Au, nano caps that are situated at a distal end from the base of a polymer sheet. Fabricated using a patented technology that can be scaled to large production volumes, our substrates enable molecular detection at ultra-low concentrations (nano-molar detection demonstrated). They provide uniform and reproduceable measurements with ultra-low background signal. A key advantage of our SERS substrates is fluorescence quenching. Raman measurements of Rhodamine B and Rhodamine 6G using Nefer Ka's substrates exhibit minimal fluorescence when excited at a wavelength of 632 nm.

 

Advantages of Nefer Ka's SERS substrates: 

  • Fluorescence quenching. The direct contact between the molecules and the metallic nano caps results in fluorescence quenching which permits the use of a shorter excitation wavelength, and thereby a stronger signal
  • Customizable to any size or geometry
  • Can be flexible if needed
  • Produced using a scalable manufacturing technique
  • Ease of use, mounted on a microscope slide
  • Uniform signal
  • Low background signal
  • Broadband range of excitation

 

 Other properties

  • Dimensions: 75 x 25 x 1.1 mm (width x height x thickness)
  • SERS active surface: 5 x 5 mm Or 10 x 10 mm (width x height)
  • Active metal: Au (Ag-based available upon request)
  • Substrate material: polymer adhered to glass
  • Sampling method: drop deposition, immersion
  • Laser wavelengths: 532 nm, 633 nm (recommended) & 785 nm
  • Stability: up to 1 year
  • Surface: hydrophilic, if drop casting it is advised to use a small volume (3 to 5 μL recommended for 5-mm substrates)
  • Sensitivity: ppm to ppb
  • Fluorescence quenching
  • Single-use substrate
  • Max laser power density: 20W/cm²

 

For large orders, please contact us at "info@neferkallc.com"

The measurements shown below highlight the advantages of using Nefer Ka's SERS substrates. 

 

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Ultra-low background signal

 Raman signal taken on and off a Rhodamine 6G analyte show the inherent low background signal using Nefer Ka's SERS substrates.

 

 

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Spatially uniform signal

Raman measurements at 3 different spots along the coffee ring of Rhodamine 6G display a uniform signal.

 

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Broad bandwidth of operation

 Excitation wavelengths  λ = 532 nm, λ = 632 nm & λ = 785 nm.

Optimal excitation wavelengths λ = 632 nm.

 

Fluorescence quenching

Nefer Ka's SERS substrates exhibit remarkable fluorescence quenching. Tested at the excitation wavelength λ = 632 nm.